This lab will help you learn the basics of modern DNA sequencing techniques and how they are used to help diagnose infectious diseases. Completing this virtual lab and answering the following questions  will allow you to post additional evidence for Portfolio Standard 2: Plan and Carry Out Scientific Investigations.

 

First, go to:  

 

http://www.hhmi.org/biointeractive/vlabs/bacterial_id/index.html or the iPad app

 

In this virtual lab you will assume the role of a lab technician in a modern molecular biology laboratory.  As such, you are responsible for providing lab results to medical doctors for use in diagnosing their patients.  Be sure to follow the steps of the procedure in order and to make use of the notes on the right side of the computer screen.  As you work through the lab, answer the following questions:

 

  1. As the medical technician in charge of this investigation, what are you trying to determine about the tissue sample provided to you?

we are try to figure out its genetic makeup

  1. How did you prepare the DNA to be used in this investigation?

by putting it in to microtubes and the putting them microtube into a hot water bath

  1. Describe how PCR is used to make copies of DNA sequences. Use the animation and notebook entries in the PCR Amplification step to guide your answer. Note that you may replay the animation as needed.

it splits and then new protiens come in to make copys of the dna

  1. Summarize the technique used to purify the PCR product.

put dna and other things into a microtube and put them in to a centrifuge a few times

  1. What is produced during the sequencing prep PCR run? Use the animation and notebook as needed in thinking through your answer.

More bacteria

  1. Describe how the automatic sequencer determines the sequences of the PCR products.

it watches the bacteria make copys and learns the products

  1. What does BLAST stand for?
 Basic Local Alignment Search Tool 
  1. What conclusions did you make using the results of the BLAST search?  Did these conclusions support a clinical diagnosis for the patient (what disease did they have)?

i said that the sample was ecoli and it was.